Τα ερευνητικά του ενδιαφέροντα του Δρ. Στυλιανού εστιάζονται κυρίως στις περιοχές της μικροσκοπίας ατομικής δύναμης, των νανο-βιοϋλικών, τις νανο-μηχανικές ιδιότητες των κυττάρων και ιστών, των συστατικών του μικροπεριβάλλοντος του καρκινικού όγκου, τις αλληλεπιδράσεις κυττάρων-βιοϋλικών, της αλληλεπίδρασης οπτικής ακτινοβολίας με βιολογικό ιστό [π.χ. λέιζερ χαμηλής ισχύος και την υπεριώδη ακτινοβολία], τις νέες τεχνικές ιατρικής απεικόνισης [π.χ. γένεση δεύτερης αρμονικής, THz απεικόνιση] και της βιοηθικής. Έχει επίσης εκτεταμένη πειραματική και εργαστηριακή εμπειρία σε τεχνικές όπως η μικροσκοπία (μικροσκοπία φθορισμού, μικροσκοπία σάρωσης ηλεκτρονίων και μη-γραμμική οπτική μικροσκοπία), οι διατάξεις λέιζερ, η φασματοσκοπία (απορρόφησης και φθορισμού) και η κυτταρική καλλιέργεια καθώς.

 

 

Current

• 01/09/2019-31/12/2021   

Cyprus Research and Innovation Foundation  - CYPRUS RESEARCH AWARD – YOUNG RESEARCHER, “The NanoMechanical Profile of Sarcoma” (CULTURE/AWARD-YR/0119, “MehanoSarcoma”), Funding: €60,000, Role: Principal Investigator

• 01/01/2019-31/12/2020   

University of Cyprus Advanced Post-doctoral Research Fellowship “NanoMechanical FingerPrints of Pancreatic Cancer” (“PACA-FingerPrints”), Funding: €74,000, Score: excellent, Role: Fellow/Principal Investigator

• 12/10/2018-11/10/2022

COST action “Correlated Multimodal Imaging in Life Sciences” (COMULIS, CA17121), Role: Cyprus Management Committee (MC) member

Past

• 06/2015-05/2017             

Horizon 2020 - Marie Skłodowska-Curie Individual Fellowship (MSCA-IF-2014-658769) MYO-DESMOPLASIA: Modulating the behaviour of cancer myofibroblasts to control tumour desmoplasia, Funding: €163,649, Score: 96.80%, Role: Fellow

• 09/2010 - 04/2014  

Scholarship “Heracleitus II” (68/1054) “Optical Radiation Effects on Collagen Topology Studied by AFM and SEM”.  The scholarship was co-financed by the European Union (European Social Fund – ESF) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: Heracleitus II. Funding: €38,750, Score: 10 out of 10, Role: Fellow

• 03/2008–01/2011                        

Scholarship for PhD students, Scholarship from Division of Electromagnetics, Electrooptics and Electronic Materials (DEEEM)/School of Electrical and Computer Engineering (ECE) /National Technical University of Athens (NTUA), Greece

• 10/2007-11/2009             

Karatheodoris, BasicResearch Program 65/1582 (National Technical University of Athens) “Non-linear microscopy investigation of quaternary structure of collagen”, Funding: €15,000, Role: Fellow

Tributes, Awards & Distinctions

2019   Young Researcher Award

CYPRUS RESEARCH AWARD – YOUNG RESEARCHER, Cyprus Research and Innovation Foundation

15-17 November, 2018     1st poster award

Louca, M., Stylianou A., Minia, A., Pliaka, V., Alexopoulos, L., Gkretsi, V., and Stylianopoulos, T. (2018) "Opposing effects of RSU-1 silencing in glioma cell invasion depending on their aggressiveness" 6th International Multithematic Bio-Medical Congress (IMBMC), Bio-Medical Scientific Cyprus (BSC), 15-17 November, 2018, Nicosia, Cyprus

02/2014 Tribute at Global Medical Discovery

The target Selection Team at Global Medical Discovery has identified the publication: “Surface nanoscale imaging of collagen thin films by Atomic Force Microscopy” as a Key Scientific Article contributing to excellence in biomedical research.

2013      “Progress of Science” award

 The publication (journal): Stylianou, Yova, D. (2013) “Surface nanoscale imaging of collagen thin films by Atomic Force Microscopy” Materials Science and Engineering C, 33 (5), 2947-2957, was award by Thomaidio Foundation, NTUA, Greece

06/2012  Tribute at Photonics Spectra

Part of the work that was performed in the BOAB Laboratory by Stylianou A. for the needs of his PhD Thesis under the supervision of prof. D. Yova was specially presented by the editor in the July 2012 issue of Photonics Spectra (Savage, L. (2012) “Nanoscale biomaterials require close observation” Photonics Spectra  46 (7) , pp. 46-50).

2012      “Progress of Science” award

The publication (conference proceeding): Stylianou, A.,Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Quantitative and Qualitative Nanoscale Characterization of Collagen Thin Films”, Proceedings of the 5th Conference on Emerging Technologies in NDT, CRC Press 2012, Chapter 65,  pp. 415-420, was award by Thomaidio Foundation, NTUA, Greece

2011      “Progress of Science” award

The publication (journal): Stylianou, A., Politopoulos, K., Kyriazi, M., Yova, D. (2011) “Combined information from AFM imaging and SHG signal analysis of collagen thin films” Biomedical Signal Processing and Control, 6 (3), 307-313, was award by Thomaidio Foundation, NTUA, Greece

 

• Panagi, M., Voutouri, C., Mpekris, F., Papageorgis,P., Martin, M., Martin, J. Demetriou, P., Pierides, C., Costeas, P., Polydorou,C., Stylianou, A, Louca, M., Kataoka, K., Cabral, H., Stylianopoulos, T. (2020) "TGF-β inhibition combined with cytotoxic nanomedicine normalizes triple negative breast cancer microenvironment towards anti-tumor immunity ", Theranostics, 10(4), 1910-1922 doi:10.7150/thno.36936.
• Gkretsi, V., Louca, M., Stylianou, A., Minadakis, G., Spyrou M. G., Stylianopoulos, T. (2020) "Inhibition of Breast Cancer Cell Invasion by Ras Suppressor-1 (RSU-1) Silencing Is Reversed by Growth Differentiation Factor-15 (GDF-15)", International Journal of Molecular Sciences, 20(1): 163, doi: 10.3390/ijms20010163
• Kontomaris, S.V., Malamou, A., Stylianou, A (2019) "Is it necessary to calculate Young’s modulus in AFM nanoindentation experiments regarding biological samples?", Micro and Nanosystems, (accepted).
• Kontomaris, S.V., Stylianou, A., Nikita, K.S. Malamou, A., (2019) "Determination of the linear elastic regime in AFM nanoindentation experiments on cells", Materials Research Express,6 (11), 115410, doi:10.1088/2053-1591/ab4f42.
• Kontomaris, S.V., Stylianou, A., Nikita, K.S. Malamou, A. (2019) "A discussion regarding the application of the Hertz contact theory on biological samples in AFM nanoindentation experiments", Micro and Nanosystems, (conditionally accepted).
• Stylianou, A, Kontomaris, S.V., Alexandratou, E., Grant, C (2019) "Atomic Force Microscopy on biological materials related to pathological conditions", Scanning, 19, 8452851, org/10.1155/2019/8452851
• Kontomaris, S.V., Alexandratou, E., Grant, C, Stylianou, A, (2019) "Atomic Force Microscopy: In Sickness and in Health" Editorial to the Special Issue Atomic Force Microscopy: In Sickness and in Health "Scanning, 19, 6149247, org/10.1155/2019/6149247
• Kontomaris, S.V., Stylianou, A., Nikita, K.S. Malamou, A., Stylianopoulos, T (2019) "A simplified approach for the determination of fitting constants in Oliver – Pharrmethod regarding biological samples", Physical Biology 16(5):056003. doi: 1088/1478-3975/ab252e.
• Stylianou A., Gkretsi, V., Louca, M., L.C. Zacharia and T. Stylianopoulos (2019) " Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts ", Journal of the Royal Society of Interface, 16 (154):20190226, org/10.1098/rsif.2019.0226
• Louca, M., Stylianou, A., Minia, A., Pliaka, V., Alexopoulos, G. L., Gkretsi, V. and Stylianopoulos, T. (2019) "Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation", Scientific Reports, 9 , 7782, org/10.1038/s41598-019-44200-8.
• Gkretsi, V., Louca, M., Stylianou, A., George Minadakis, G., Spyrou, G.M and Stylianopoulos, T. (2019) "Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by Growth Differentiation Factor-15 (GDF-15)", International Journal of Molecular Sciences, 20 (1), doi:10.3390/ijms20010163
• Stylianou A., M. Lekka and T. Stylianopoulos. AFM Assessing of Nanomechanical FingerPrints for Cancer Early Diagnosis and Classification: from single cell to tissue level. Nanoscale, doi: org/1039/C8NR06146G
• Louca, M., Stylianou, A., Minia, A., Pliaka, V., Alexopoulos, G. L., Gkretsi, V. and Stylianopoulos, T. " Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation", Scientific Reports (accepted).
• Stylianou, A., Gkretsi, V. and Stylianopoulos, T. (2018) "Transforming Growth Factor-β modulates Pancreatic Cancer Associated Fibroblasts cell shape, stiffness and invasion", Biophysica and Biochemica Acta-General Subjects, 1862(7),1537-1546, org/10.1016/j.bbagen.2018.02.009
• Stylianou, A., Gkretsi, V. and Stylianopoulos, T. (2018) "Atomic Force Microscopy Nano-Characterization of 3D Collagen Gels with Tunable Stiffness", MethodsX, 5, 503-513, org/10.1016/j.mex.2018.05.009
• Kontomaris, S.V, Stylianou, A., Malamou, A, Stylianopoulos, T. (2018) "A discussion regarding the approximation of cylindrical and spherical shaped samples as half spaces in AFM nanoindentation experiments", Materials Research Express, 5 (8), org/10.1088/2053-1591/aad2c9
• Stylianou, A. (2017) "Atomic Force Microscopy for Collagen-Based Nanobiomaterial", Journal of Nanomaterials, id. 9234627, doi:10.1155/2017/9234627, (invited)
• Kontomaris, S.V and Stylianou, A. (2017) "Atomic Force Microscopy for University Students, Application in Biomaterials" European Journal of Physics, 38 (3), org/10.1088/1361-6404/aa5cd6
• Gkretsi, V., Stylianou, A. and Stylianopoulos, T. (2017) " Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPA)" Experimental Cell Research, 352(2): 281-292, doi: 10.1016/j.yexcr.2017.02.019
• Gkretsi, V., Stylianou, A., Louca, M. and Stylianopoulos, T. "Identification of Ras Suppressor-1 (RSU-1) as a potential breast cancer metastasis biomarker using a three-dimensional in vitro approach" Oncotarget, 8(16):27364-27379, doi: 10.18632/oncotarget.16062
• Stylianou, A, Talias, M.A " Big data in healthcare: a discussion on the big challenges " (2017), Health and Technology, 7 (1), 97-107, doi:10.1007/s12553-016-0152-4
• Stylianou, A. and Stylianopoulos, T. (2016) "Atomic Force Microscopy Probing of Cancer Cells and Tumor Microenvironment Components" BioNanoScience, 6 (1), 33-46, doi:10.1007/s12668-015-0187-4 (invited)
• Kontomaris, S.V, Stylianou, A. Yova, D (2016) "Investigation of the mechanical properties of collagen fibrils under the influence of low power red laser irradiation", ‘Special issue on Emerging Trends in Biomedical Physics and Engineering’ , Biomedical Physics and Engineering Express, 2 0640, org/10.1088/2057-1976/aa5195
• Kontomaris, S.V., Yova, D., Stylianou, A. and Politopoulos, K. (2015) "The Significance of the Percentage Differences of Young’s Modulus in the AFM Nanoindentation Procedure" Micro and Nanosystems, 7 (2), 86-97, doi: 10.2174/1876402908666151111234441
• Gkretsi, V., Stylianou, A., Papageorgios, P., Polydorou, C. and Stylianopoulos, T. (2015) "Remodeling components of the tumor microenvironment to enhance cancer therapy" Frontiers in Oncology, 5 (214), doi: 10.3389/fonc.2015.00214
• Stylianou, A., Yova, D., Alexandratou, E. (2015) "Atomic Force Microscopy Investigation of the Interaction of Low-Level Laser Irradiation of Collagen Thin Films in Correlation with Fibroblast Response" Lasers in Medical Science, 30 (9), doi:2369-2379 10.1007/s10103-015-1823-5
• Stylianou, A., Kontomaris S.V, Yova D. (2014) "Assessing Collagen Nanoscale Thin Films Heterogeneity by AFM Multimode Imaging and Nanoindetation for NanoBioMedical Applications", Special Issue on Bio-micro and bio-nano technologies, Micro and Nanosystems, 6 (2), 95-102,  doi:10.2174/187640290602141127114448
• Kontomaris S.V, Yova D., Stylianou A., Balogiannis G. (2014) "Influence of UV Irradiation on Collagen Fibrils Using AFM Imaging and Nanoindentation Methods", Scanning, 37 (23), 101-111, doi:10.1002/sca.21185
• Stylianou, A., Yova, D., Alexandratou, E. (2014) "Investigation of the Influence of UV Irradiation on Collagen Thin Films by AFM Imaging Microscopy", Materials Science and Engineering: C, Materials for Biological Applications, 45, 455–468, doi: 0.1016/j.msec.2014.09.006
• Stylianou, A, Talias, MA (2014) "The 'magic light': a discussion on laser ethics", Science and Engineering Ethics, 21 (4), 979-998, doi: 10.1007/s11948-014-9566-4
• Stylianou, A, Talias, MA. (2013) "Nanotechnology-supported THz medical imaging" F1000 Research, 2:100, 2 (1), 1-14, doi: 10.12688/f1000research.2-100.v1
• Stylianou, A., Yova, D., Alexandratou, E. (2013) "Nanotopography of collagen thin films in correlation with fibroblast response", Journal of Nanophotonics, Special Section: Nanostructured Thin Films: Fundamentals and Applications, 7 (1), art no. 073590, doi: 10.1117/1.JNP.7.073590
• Stylianou, A., Yova, D. (2013) "Surface Nanoscale Imaging of Collagen Thin by Atomic Force Microscopy", Materials Science and Engineering: C, Materials for Biological Applications, 33 (5), 2947-2957, doi:10.1016/j.msec.2013.03.029
• Stylianou, A., Politopoulos K, Kyriazi M, Yova D. (2011) "Combined information from AFM imaging and SHG signal analysis of collagen thin films", Biomedical Signal Processing and Control, 6 (3), 307-313, doi:10.1016/j.bspc.2011.02.006